PREPARING EUGREGARINE PARASITES AND THEIR CRICKET HOST ACHETA DOMESTICUS AS A MODEL FOR GREGARINE INFECTION STUDIES

Preparing eugregarine parasites and their cricket host Acheta domesticus as a model for gregarine infection studies

Preparing eugregarine parasites and their cricket host Acheta domesticus as a model for gregarine infection studies

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The increasing global demand for house crickets (Acheta domesticus) necessitates effective health measures.Despite their abundance, the effects of gregarines on these hosts remain underexplored.We present a method for controlled gregarine infection and maintenance of gregarine-free cricket populations.This system, adapted from prior Bike Units - Electric Bikes work, is essential for experiments on gregarine infection dynamics, providing insights into parasite evolution and host-parasite interactions within the Apicomplexa group.This protocol includes Headband rearing and maintenance of gregarine-free cricket populations for experimental purposes, gregarine production for oocyst solution and cricket infection, and gregarine infection assessment.

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